Which restriction enzyme is sensitive to methylation

Each sample value is a mean deviation of values from three independent experiments. The absence of a 0. The digestion analysis of the mock-methylated fragment with Bst Z17I yielded 5. The present investigation, therefore, provides another restriction endonuclease, Bst Z17I, for the analysis of cellular DNA methylation levels.

I acknowledge the valuable advice and help from Prof. Rolf Ohlsson. I thank an anonymous reviewer of a previous version of this manuscript.

Kessler C. Manta V. Gene 92 , 1 — Google Scholar. Kanduri C. Holmgren C. Pilartz M. Franklin G. Kanduri M. Liu L. Ginjala V. Ulleras E. Mattsson R. Ohlsson R. Raman R. Nucleic Acids Res. Bull L. Hewitt J. Cox D. Myers R. Restriction enzyme. Recognition sequence 1. Methylase influencing restriction cleavage efficiency and its recognition sequence. Cleavage sites blocked by methylation 2.

Aat II. CG methylase 5m CG. Acc I. Acc II. Acc III. Afa I. GTA 5m C 3,4. Afl II. Alu I. Aor 13HI. Aor 51HI. Apa I. Bal I. Bcn I. Bgl I. Bgl II. Bln I. Cla I. Cpo I. Eae I. Fba I. Fok I. Fse I. Hae II. Hae III.

Hha I. Hinc II. Hinf I. GANT 5m C 3. Hpa I. Search Menu. Article Navigation. Close mobile search navigation Article Navigation. Volume Article Contents Acknowledgements. Oxford Academic.

Google Scholar. Rajiva Raman. Select Format Select format. Permissions Icon Permissions. Figure 1. Open in new tab Download slide. Search ADS. Issue Section:. Download all slides.

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